University of Wisconsin-Madison

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Instrumentation

NMR Spectroscopy

  • Varian Unity-Inova 400 MHz NMR Spectrometer
    • 2 RF channels
    • z-axis pulsed field gradient equipped (hardware and probe)
    • Varian quad-nucleus probe, capable of 1H and 19F detection (high band) and 13C and 31P detection (low band), plus X (low band) tunable between 15N and 31P (e.g., 7Li, 15N, 17O, 23Na, 29Si, 77Se, 119Sn)
  • Varian Unity-Inova 500 MHz NMR Spectrometer
    • 3 RF channels
    • Waveform generators
    • z-axis pulsed field gradient equipped (hardware and all probes)
    • Varian triple-resonance, inverse-detection probe, capable of 1H{13C/X} experiments, with X tunable between 15N and 31P
    • Protasis/MRM inverse-detection, capillary probe, capable of 1H{13C} experiments, with a 5 µL sample volume (for mass-limited samples)
    • Nalorac quad-nucleus probe, capable of 1H and 19F detection (high band) and 13C and 31P detection (low band)

Mass Spectrometry

  • Bruker MaXis Ultra-High Resolution Quadrupole Time-of-Flight MS
    • Dual ion funnel design for maximal sensitivity
    • Capable of resolution >65,000 FWHM for MS and MS/MS
    • Mass Accuracy <1 ppm with internal calibration
    • Maximum acquisition rate 20 Hz
  • Bruker Amazon ETD Ion Trap
    • Capable of collision induced dissociation and electron transfer dissociation (for post-translation modification characterization)
    • MSn using CID, ETD or different combinations
    • Multiple acquisition rates for optimizing speed or resolution
    • Zero delay positive/negative switching
  • Bruker UltraFlex MALDI-ToF/ToF 
    • 2 KHz NdYAG, Smart Beam II laser capable of beam focus to 10 microns with no pixel overlap
    • MALDI-MS Imaging through FlexImaging software
    • Resolution of 40,000 FWHM
    • Mass Accuracy <1 ppm with internal calibration
    • Linear and Reflectron modes
  • Thermo Orbitrap Elite with ETD-High Resolution Orbitrap MS
    • Hybrid mass spectrometer with Velos linear ion trap and Orbitrap detectors
    • Smaller diameter Orbitrap cell allows resolution up to 240,000 FWHM @ 400 m/z
    • Mass Accuracy <1 ppm with internal calibration
    • Multiple fragmentation mechanisms available, CID, HCD and ETD
  • Thermo Orbitrap Q-Exactive MS
    • Quadrupole-Orbitrap configuration allows broader mass range, 50-6000 m/z
    • Smaller diameter Orbitrap cell allows resolution up to 140,000 FWHM @ 200 m/z
    • Mass Accuracy <1 ppm with internal calibration
    • Capable of fast polarity switching
    • Dynamic range >5000 in 1 scan
  • AB Sciex Q-Trap 5500
    • Turbo ion spray source for maximal sensitivity
    • Q3 linear ion trap allows traditional triple quad MRM or MSn
    • Automated compound optimization facilitates method development
  • Waters Acquity UPLC System
    • Capable of pressures to 15,000 psi
    • Flow rates from 0.1-2.0 ml/min
    • Photodiode array UV detector
    • Peak widths <6 sec
    • Can couple to Bruker and AB Sciex spectrometers
  • Waters Nano-Acquity UPLC System
    • Capable of pressures to 15,000 psi
    • Splitless flow rates from 0.2-200.0 microliters/min
    • Can be coupled to Bruker instruments
  • Agilent 1100 HPLC-MSD SL Ion Trap Mass Spectrometer
    • Binary pump HPLC with microvacuum degaser
    • Temperature controlled, 100-vial autosampler
    • Dual column oven with a column switching valve
    • UV/Vis diode array detector
    • Orthogonal pneumatically assisted electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources

 Decommissioned

  • IonSpec ProMALDI FT Mass Spectrometer
  • Agilent 1100 HPLC-MSD VL Quadrupole Mass Spectrometer

Spectrophotometry

  • Bruker Equinox 55/S FT–IR Spectrophotometer
    • Conventional and step scanning modes for time-resolved spectroscopy
    • Fast scan option
    • Dual sources for Near-IR and Mid-IR spectral ranges
    • DTGS, liquid-nitrogen-cooled MCT, and NIR Ge diode detectors
    • Various sampling accessories are available
  • Thermo AB Series 2 Luminescence Spectrophotometer
    • Excitation or emission wavelength scans, synchronous scans, time assays and 3D measurement modes
    • Intracellular probe measurement mode
    • Fast-slewing 1200 lines/mm grating monochromators
    • Temperature-controlled cuvette holder
  • ISS Koala Fluorescence Spectrophotometer
    • Excitation or emission wavelength scans, synchronous scans and time assays
    • Photon counting mode
    • Glan-Thompson prism polarizers for polarization and anisotropy measurements
    • 1200 lines/mm grating monochromators
    • Temperature-controlled dual sample turret cuvette holder with integrated stirring accessory
  • Hitachi F–3010 Fluorescence Spectrophotometer
    •  Excitation or emission wavelength scans, synchronous scans, time assays and quantitative measurement modes
    • 900 lines/mm grating monochromators
    • Temperature-controlled cuvette holder with integrated stirring accessory
  • Olis Aminco DW-2 UV/Vis Spectrophotometer
    • Two operational modes
    • Conventional double beam spectrophotometer mode
    • Double monochromator/dual wavelength mode for highly scattering samples
    • Wavelength scans (200 nm to 850 nm spectral range) and time assays
    • Temperature-controlled cuvette holder
  • Perkin–Elmer 241 Polarimeter
    • Sodium and mercury lamp sources
    • Temperature-controlled cell having 1 mL volume and 1 dm pathlength